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Section A - Cells and macromloecules1

A1 Cellular Classification1

A2 Subcellular organelles4

A3 Macromolecules7

Abbreviations9

F4 Recombimation9

A4 Large macromolecular assemblies11

Preface to the second edition11

Preface to the first edition13

Section B - Protein structure15

B1 Amino acids15

B2 Protein structure and function18

B3 Protein analysis25

C1 Nucleic acid structure31

Section C - Properties of nucleic acids31

C2 Chemical and physical properties of nucleic acids38

C3 Spectroscopic and thermal properties of nucleic acids42

C4 DNA supercoiling45

Section D - Prokaryotic and eukaryotic chromosome structure49

D1 Prokaryotic chromosome structure49

D2 Chromatin structure51

D3 Eukaryotic chromosome structure56

D4 Genome complexity61

D5 The flow of gengtic information66

E1 DNA replication：an overview71

Section E - DNA replication71

E2 Bacterial DNA replication76

E3 The cell cycle80

E4 Eukaryotic DNA replication84

Section F- DNA damage,repair and recombimation89

F1 Mutagenesis89

F2 DAN damage93

F3 DAN repair96

Section G - Gene manipulation103

G1 DAN cloning：an overview103

G2 Preparation of plasmid DNA108

G3 Restriction dnzymes and electriphoresis111

G4 Ligation.transformation and analysis of recombinants116

Section H - Cloning vectors123

H1 Desing of plasmid vectors123

H2 Bacteriophage vectors127

H3 Cosmide,YACs and BACs132

H4 Eukaryotic vectors137

Section I - Gene libraries and screening143

I1 Genomic libraries143

I2 cDNA libraries146

I3 Screening procedures151

Section J - Analysis and uses of cloned DNA155

J1 Charactrization of clones155

J2 Nucleic acid sequencing160

J3 Polymeraes chain reaction165

J4 Organization of clonen genes170

J5 Mutagenesis of cloned genes174

J6 Applications of cloning178

Section K - Transcription in prokaryotes183

K1 Basic principles of transcription183

K2 Escherichia coli RNA poly merase186

K3 The E. coli a 70 promoter188

K4 Transcription,initiation,elongation and etrmination191

Section L - Regulation of transcrlption in prokaryotes197

L1 The lac operon197

L2 The trp operon201

L3 Transcriptional regulation by alternative or factors205

Section M - Transcription in eukaryotes209

M1 The three RNA polymerases:characterization and function209

M2 RNA polⅠ genes:the ribosomal repeat211

M3 RNA pol Ⅲ genes： 5S and tRNA transcription215

M4 RAN PolⅡgenes;promoters and enhancers219

M5 General transcripton factors and RNA Pol Ⅱinitiation221

Section N - Regulation of transcription in eukaryotes225

N1 Eukaryotic transcription facrots225

N2 Examples of transcriptional regulation231

Section O - RNA processing and RNPs237

O1 rRNA processing and ribosornes237

O2 tRNA processing,RNase P and ribozymes243

O3 mRNA processing, hnRNPs and snRNPs246

O4 Alternative mRNA processing252

Section P - The genetic code and tRNA255

P1 The genetic code255

P2 tRNA structuer and function259

Section Q - Protenin symthesis265

Q1 Aspects of protein symthesis265

Q2 Mechanism of protein symthesis269

Q3 Initiation in eukaryotes275

Q4 Translational control and post-translational events279

R1 Introduction to viruese283

Section R - Baclexiophages and eukaryotic viruses283

R2 Bacterophages287

R3 DNA viruses292

R4 RNA viruses296

Section S - Tumor viruses and oncogenes301

S1 Oncogenes found in turnor viruses301

S2 Categories of on cogenes305

S3 Tumor suppressor genes308

S4 Apoptosis312

Futrher reading317

Multiple choice questions321

Ansers337

Index339